Lack of plasmids in Streptomyces hydrogenans
نویسندگان
چکیده
منابع مشابه
Testosterone metabolism in streptomyces hydrogenans.
After cultivation of Streptomyces hydrogenans in the presence of 3H-labelled testosterone, radioactive steroids were extracted separately from the cytosolic, ribosomal and cell wall-membrane fraction of the cells and from the culture medium, respectively. The separation of the steroids was performed by one- and two-dimensional thin layer chromatography (TLC). The identification of the main meta...
متن کاملProperties of an antiserum against Streptomyces hydrogenans 20beta-hydroxysteroid dehydrogenase.
Antiserum against crystallized 20beta-hydroxysteroid dehydrogenase from Streptomyces hydrogenans was used for different immunodiffusion and immunoprecipitation tests to quantify the bacterial enzyme in cell-free supernatants of the microorganism. After immunoprecipitation and gel electrophoresis the molecular weight of the subunits of 20beta-hydroxysteroid dehydrogenase was calculated to be 27 ...
متن کاملIsolation and characterization of plasmids from Streptomyces.
In view of the importance of actinomycetes in the production of antibiotics, it would be useful to find plasmids in these organisms in order to construct molecular breeding systems. Since the first discovery of plasmid involvement in antibiotic production", several plasmids associated with the production of antibiotics have been found in Streptomyces (references are listed in AKAGAWA et al.-')....
متن کاملStudies on the transport of anions and zwitterions of acidic amino acids in Streptomyces hydrogenans.
In Streptomyces hydrogenans, acidic amino acids are taken up either as anions by a specific transport system or as zwitterions via a nonspecific one. Variations in the zwitterion concentration caused by changes in pH influence the uptake and exchange diffusion by the nonspecific system. Differences in pH-optima for L-glutamate and L-aspartate transport are due to the different pK2-values of the...
متن کاملPurification and characterization of a 3,17 beta-hydroxysteroid dehydrogenase from Streptomyces hydrogenans.
3,17 ß-Hydroxysteroid dehydrogenase has been enriched and purified from cytosol o f Strepto myces hydrogenans. After ammonium sulfate precipitation and filtration on Sephadex G-100 the enzyme was finally purified by preparative gel electrophoresis and DEAE-Sephadex A-50 chro matography. Polyaciylam ide gel electrophoresis in the presence o f sodium dodecylsulfate gave a single band o f mobili...
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ژورنال
عنوان ژورنال: FEMS Microbiology Letters
سال: 1988
ISSN: 0378-1097
DOI: 10.1016/0378-1097(88)90114-0